This sign means:

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The lane is restricted to authorized vehicle.
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You must change lanes immediately for opposing traffic.
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The road ahead is closed.
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You are attempting to enter a one-way street from the wrong direction.
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Explanation
The "Do Not Enter" sign prohibits drivers from entering a roadway from the wrong direction, typically indicating a one-way street or highway on- or off-ramps. If you encounter this sign while driving, stop immediately in a safe manner and turn around legally when possible. This sign is often paired with a "Wrong Way" sign to emphasize the danger. Violating this sign creates head-on collision risks and may result in traffic citations.
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