The Do Not Enter sign is used to prevent:

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Vehicles from entering bike lanes.
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Vehicles from entering one-way streets and highway ramps in the wrong direction.
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Pedestrians from entering highways.
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Large vehicles from entering narrow bridges.
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Explanation
The "Do Not Enter" sign is used to prevent drivers from entering one-way streets or highway ramps in the wrong direction - something that could lead to a serious head-on collision. Never ignore or drive past a "Do Not Enter" sign, as doing so creates an extremely dangerous situation.
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